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KMID : 0381919980280040591
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Korean Journal of Microscopy
1998 Volume.28 No. 4 p.591 ~ p.601
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Irradiation in Central Nervous System and Recovery Effect: Action of Antioxidants and NMDA-receptor Antagonists
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Mun Yeun-Ja
Park Seung-Taeck
Choi Min-Kyu
Jeong Dong-Hyeok
Moon Sung-Rock
Chung Yeun-Tai
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Abstract
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The neurotoxic effects of radiation have been studied in NSC-34 hybrid cells derived from embryonic mouse spinal cord cells. NSC-34 cells irradiated at 25Gy were decreased the cell viability in a time and dose dependent manner. The decrease in cell viability induced by the irradiation was blocked by catalase. Antagonists of the N-methyl-D-aspartate (NMDA) receptor, including D-2-amino-5-phosphonovaleric acid (APV) and chlorokynurenic acid (CKA), similarly blocked radiational induced in cell viability. We performed morphological analysis of light and electron microscope. NSC-34 cells irradiated at 25Gy were decreased the cell density and increased lysosomes and vacuoles in the cytoplasm. Especially chromatin modification was observed. These results indicated that radiation was involved in the oxidant-initiated neurotoxicity and the compounds catalase, APV and CKA were shown to be neuroprotective against radiation.
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KEYWORD
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Neurotoxic, Catalase, NMDA-receptor antagonist, Rdiation, Electron microscope
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